What does ABC stand for in IHC detection?

What does ABC stand for in IHC detection?

• A. Avidin-Biotin Complex
• B. Antibody-Blocking Complex
• C. Antigen-Bridge Complex
• D. Avian-Bovine Conjugate

Answer: (A)

In IHC detection, the ABC system refers to Avidin-Biotin Complex. This method uses a biotinylated detection component that binds to the primary antibody, followed by an avidin or streptavidin enzyme conjugate that attaches to the biotin. The extremely strong biotin-avidin interaction, along with the multiple binding sites on avidin, results in efficient enzyme localization and signal amplification, making the stain more sensitive. The other options aren’t standard terms for this detection approach, so they don’t describe how the signal is generated.

In IHC detection, the ABC system refers to Avidin-Biotin Complex. This method uses a biotinylated detection component that binds to the primary antibody, followed by an avidin or streptavidin enzyme conjugate that attaches to the biotin. The extremely strong biotin-avidin interaction, along with the multiple binding sites on avidin, results in efficient enzyme localization and signal amplification, making the stain more sensitive. The other options aren’t standard terms for this detection approach, so they don’t describe how the signal is generated.