In the ABC method, Secondary antibodies are conjugated with Biotin.

In the ABC method, Secondary antibodies are conjugated with Biotin.

• A. True
• B. False
• C. Not specified
• D. Not applicable

Answer: (A)

The ABC method relies on biotinylated secondary antibodies. After the primary antibody binds the target, the secondary antibody that recognizes the primary is conjugated with biotin. Then an avidin- or streptavidin-enzyme complex is applied, which binds to the biotin on the secondary antibody. This setup amplifies the signal because avidin has multiple binding sites and multiple biotin interactions can occur, leading to strong enzymatic labeling and visualization. While there are variations that use direct enzyme labeling or different detection steps, the classic ABC approach centers on biotinylated secondary antibodies, making the statement true.

The ABC method relies on biotinylated secondary antibodies. After the primary antibody binds the target, the secondary antibody that recognizes the primary is conjugated with biotin. Then an avidin- or streptavidin-enzyme complex is applied, which binds to the biotin on the secondary antibody. This setup amplifies the signal because avidin has multiple binding sites and multiple biotin interactions can occur, leading to strong enzymatic labeling and visualization. While there are variations that use direct enzyme labeling or different detection steps, the classic ABC approach centers on biotinylated secondary antibodies, making the statement true.